Description
Pigs have been shown to be key hosts for the generation of pandemic influenza viruses including the one that caused the 2009 H1N1 pandemic. Since 2016, a recently identified H1N1 swine flu virus genotype, the genotype 4 reassortant Eurasian avian-like (G4 EA) H1N1 virus, has rapidly spread and become the predominant genotype in the surveilled pig populations in China. The G4 EA H1N1 swine flu virus also demonstrated human infectivity and has the potential to become a pandemic virus. ScienCell's G4 EA H1N1 Swine Flu Virus RT-qPCR Detection Kit (G4EASF) is designed to detect the presence of influenza A viruses and the newly emerged genotype G4 EA H1N1 Swine Flu virus in extracted RNA samples. Three primer/probe sets are included in the kit. Two of them (Cat #7068-InfA and 7068-G4EA) target a universal region shared by all influenza A viruses and a region specific to G4 EA H1N1 virus, respectively. The human ACTB gene primer/probe set (Cat #7068-ACTB) targets the human β-actin (ACTB) housekeeping gene, which serves as an internal control to assess specimen quality. All primer/probe sets are verified to possess high specificity and efficiency near 100% under recommended PCR conditions. In addition, a non-infectious positive control (Cat #7068-Pos) and nuclease-free water are included in the kit. The positive control (Cat #7068-Pos) consists of non-infectious viral RNA fragments and serves to ensure reagents and instruments are working properly. Please refer to Tables 4 and 5 for results interpretation